Research Paper Volume 12, Issue 14 pp 13979—13990

Senescence-like phenotype in post-mitotic cells of mice entering middle age

Figure 6. pH-dependent (pH 4 to pH 7) β-gal activity in frozen sections of 9 months old C57/Bl6J mouse intestine. Nuclear Fast Red was used for counterstaining. At pH 6, specific for SA- β-gal, bluish color from β-gal activity is evident specifically in cells located basally in the intestinal crypts. Representative images from 3 different mice are shown.