Figure 8. (A) Immunofluorescent staining of senescence markers of 9 months old C57/Bl6J mouse choroid plexus. Images display H3K4me3 (blue), β-gal (purple), p21 (green), DPP4 (cyan) p16 (red) and DAPI-stained nuclei (white) fluorescence signals. The corresponding multichannel overlaid images are shown in the right column. All these markers show an increased localization and expression in choroid plexus cells. Representative images from 3 different mice are shown. (B) Frequency of positive cells for each marker as in (A), indicated in %.