Figure 2. Effects of NF-κB p65 silencing on heart failure in rats. (A) NF-κB p65 mRNA expression after sh-RNA treatment; (B) NF-κB p65 protein expression in heart tissues; (C) Ventricular mass index; (D) CVF determined by Masson’s trichrome staining (100 ×); (E) Apoptosis determined by TUNEL staining (200 ×); *p < 0.05 vs. model + sh-NC group; # indicates lowest NF-κB p65 mRNA expression. Data are expressed as mean ± standard deviation. A comparison of data from 2 groups was performed by an unpaired t test, while 3 or more groups were performed by one-way analysis of variance (ANOVA) and Tukey's post hoc test. N= 12.