Research Paper Volume 12, Issue 16 pp 16211—16223

Figure 1. Mst1 promotes apoptosis in oxidative stress-induced RA-FLSs. (A) QRT-PCR analysis shows Mst1 mRNA levels in the control and H₂O₂-treated RA-FLSs. (B) Representative immunofluorescence images show Mst1 protein expression in control and H₂O₂-treated RA-FLSs (C) Quantitative estimation of relative Mst1 protein levels in control and H₂O₂-treated RA-FLSs based on the immunofluorescence assay. (D) MTT assay results show the viability of control and Mst1 knockdown RA-FLSs treated with or without H₂O_2. (E) ELISA assay results show caspase-3 activity in the control and H₂O₂-treated RA-FLSs. (F) Representative images show TUNEL staining of the control and H₂O₂-treated RA-FLSs. (G) Quantification of percent TUNEL-positive (apoptotic) cells in the control and H₂O₂-treated RA-FLSs. Note: RA-FLSs were treated with 0.3 mM H₂O₂ for 6 h; *P<0.05.