Figure 3. Mst1-dependent Sirt1 downregulation in oxidative stress-induced RA-FLSs. (A) QRT-PCR assay shows Sirt1 mRNA levels in control and H2O2-treated RA-FLSs. (B, C) Immunofluorescence staining shows Sirt1 protein levels in the control and Mst1-silenced RA-FLSs treated with or without H2O2. (D, E) QRT-PCR assay shows Sirt3 and Sirt6 mRNA levels in the control and H2O2-treated RA-FLSs. Note: RA-FLSs were treated with 0.3 mM H2O2 for 6 h; *P<0.05.