Research Paper Volume 12, Issue 16 pp 16211—16223

Figure 5. Mst1 reduces Sirt1 expression in oxidative stress-induced RA-FLSs by inhibiting the AMPK signaling pathway. (A) QRT-PCR assay results show the Sirt1 mRNA levels in the control and Mst1-knockdown RA-FLSs, pretreated with or without compound C, and treated with or without 0.3 mM H₂O₂ for 6 h. Compound C (CC) is an antagonist of the AMPK pathway. (B, C) Immunofluorescence staining results show Sirt1 protein levels in the control and Mst1-knockdown RA-FLSs, pretreated with or without compound C, and treated with or without 0.3 mM H₂O₂ for 6 h. (D, E) QRT-PCR analysis shows Sirt3 and Sirt6 mRNA levels in the control and Mst1-knockdown RA-FLSs, pretreated with or without compound C, and treated with or without 0.3 mM H₂O₂ for 6 h. *P<0.05.