Figure 1. SirT3 and AMPK are downregulated in response to LPS-induced septic cardiomyopathy. (A, B) RNA was isolated, and the transcription of SirT3 and AMPK was remeasured. (C, D) ELISA was used to analyze the activities of SirT3 and AMPK in response to LPS treatment. (E) MTT assay was used to detect cardiomyocyte viability under LPS treatment. (F, G). TUNEL staining for apoptotic cardiomyocytes. Lentivirus-loaded SirT3 (SirT3-OE) and AMPK agonist (AICAR) were incubated with cardiomyocytes in the presence of LPS. Then, the number of TUNEL-positive cardiomyocytes was determined. *P < 0.05.