Research Paper Volume 12, Issue 16 pp 16224—16237

SirT3 activates AMPK-related mitochondrial biogenesis and ameliorates sepsis-induced myocardial injury

Figure 4. Mitochondrial biogenesis is increased by SirT3 via the AMPK pathway. (AC) RNA was isolated from cardiomyocytes, and then transcription of mitochondrial biogenesis parameters was determined. Lentivirus-loaded SirT3 (SirT3-OE) and AMPK agonist (AICAR) were incubated with cardiomyocyte before LPS treatment. Compound C (CC), an antagonist of AMPK, was used to inhibit the activation of AMPK in SirT3-OE–transfected cardiomyocytes. (DF) Immunofluorescence assay for Nrf2 and PGC1α in the presence of LPS. *P < 0.05.