Research Paper Volume 12, Issue 16 pp 16255—16269

Figure 1. Glucose, cholesterol, and Δ⁷-dafachronic acid promote neuronal Q-cell divisions in L1-arrested worms. (A) Normal L1-arrested wild-type worms. The wild-type L1-arrested worms only have two Q-cells (QR/L). Wild-type L1-arrested worms treated with 20 mg/mL glucose (B, E), 1 mg/mL cholesterol (C, F), or 1 μg/mLΔ⁷-dafachronic acid (D, G). Q-cell divisions occurred in these worms, as at least four Q-cells were observed under these conditions. See the dosage treatment results in Supplementary Figure 1, and the Q-cell final descendants test in Supplementary Figure 2. (H) Q-cell divisions induced by glucose or insulin treatments can be suppressed by daf-2 mutants. (I) Q-cell divisions induced by cholesterol treatment can be suppressed by daf-9 and daf-36 mutants. White bar: 50 μm. Data are the average of three independent experiments. Error bars: Standard Deviation (SD). ***: P<0.001.