Figure 6. Cetuximab and miR-155-5p antagomir combination treatment induces pyroptosis in TNBC cells. EGFR-overexpressed MDA-MB-468 cells were treated with cetuximab (10 nM) and/or the miR-155-5p antagomir (10 nM) for 72 h. (A) Expression levels of GSDME-N, cleaved caspase-1, and p-EGFR in cells were detected by western blotting. (B, C) The relative expression levels of GSDME-N and cleaved caspase-1 were normalized to β-actin. (D) The relative expression level of p-EGFR in cells was normalized to EGFR. (E, F) The relative fluorescence expression levels were quantified by GSDME-N and DAPI staining. (G, H) Levels of IL-1β and IL-18 in cells were detected by RT-qPCR. (I) The ultrastructure of the cells was observed under a transmission electron microscope (TEM). Red arrowheads indicate the large bubbles emerging from the plasma membrane. **P < 0.01 compared with the control group. ##P < 0.01 compared with cetuximab the 10 nM group.