Research Paper Volume 12, Issue 18 pp 18209—18220

piR-19166 inhibits migration and metastasis through CTTN/MMPs pathway in prostate carcinoma


Figure 7. Rescue assays detected the effects of CTTN suppressed by piR-19166 in metastasis. (A, B) RNA and protein levels of CTTN were detected respectively by qRT-PCR and western blot in PC3 cells with the presence of CTTN overexpression or vector control and si-CTTN or siRNA control. (C, D) Overexpression of CTTN rescued the biologic effects via piR-19166-induced, whereas knockdown of CTTN simulated the effects associated with piR-19166 through cell migration in PC3. Error bars represent the mean ± SD of three independent experiments. *P <0.05, ** P < 0.01, ***P < 0.001.