Research Paper Volume 12, Issue 16 pp 16368—16389

Flavonoids from Rosa roxburghii Tratt prevent reactive oxygen species-mediated DNA damage in thymus cells both combined with and without PARP-1 expression after exposure to radiation in vivo


Figure 1. FRT protected against radiation damage through PARP-1 both in vivo and in vitro. (A) PCR was used to identify PARP-1 gene in KO mice; (B) Animal model of radiation injury induced by 6 Gy irradiation in mice; (C) Mice were administered FRT orally for 4 days prior to irradiation. The thymus was harvested 4 days after irradiation and the tissue appearance observed by using a digital camera (n=5). (D) Thymus cells were prepared from WT mice and KO mice and irradiated with a dose of 6 Gy. FRT of 50 and 100 μg/mL were provided to cells 2 h prior to irradiation. The CCK-8 method was used to measure cell viability 6 h after irradiation. Data was expressed as mean ±SD, n=5. (** P<0.01 compared with normal group; ## P< 0.01 compared with radiation group).