Figure 3. Effects of PRDX2 knockdown on the cell cycle. (A) Cell proliferation was markedly inhibited after PRDX2 downregulation as determined by CCK-8 assay. (B) Flow cytometry cell-cycle analysis showed that sh-PRDX2 induces cell-cycle arrest in HT29 and HCT116 cells. (C) Representative western blot images of the effect of sh-PRDX2 on the expression levels of PRDX2, P21, and P27. β-actin was used as a loading control. The data are shown as the mean ± SD of three experiments. *P < 0.05, **P < 0.01, ***P < 0.001 compared with the control group.