Research Paper Volume 12, Issue 16 pp 16390—16409

PRDX2 removal inhibits the cell cycle and autophagy in colorectal cancer cells

Figure 3. Effects of PRDX2 knockdown on the cell cycle. (A) Cell proliferation was markedly inhibited after PRDX2 downregulation as determined by CCK-8 assay. (B) Flow cytometry cell-cycle analysis showed that sh-PRDX2 induces cell-cycle arrest in HT29 and HCT116 cells. (C) Representative western blot images of the effect of sh-PRDX2 on the expression levels of PRDX2, P21, and P27. β-actin was used as a loading control. The data are shown as the mean ± SD of three experiments. *P < 0.05, **P < 0.01, ***P < 0.001 compared with the control group.