Figure 4. Knockdown of PRDX2 suppresses CRC cell autophagy flux. (A) Immunofluorescence images of GFP-labeled LC3B staining in sh-PRDX2 and control CRC cells. (B) NC and sh-PRDX2 cells were transfected with GFP-RFP-LC3 plasmids. (C) Sh-PRDX2 and control CRC cells were analyzed by transmission electron microscopy. Arrows indicate autophagosomes. (D) Western blot of autophagy-related proteins (LC3B, SQSTM1/P62, Beclin 1) in sh-PRDX2 and control CRC cells. (E) The ratio of LC3 II/I. The data are shown as the mean ± SD of three experiments. *P < 0.05, **P < 0.01, ***P < 0.001 compared with the control group.