Figure 5. Metformin enhanced relative mRNA expression of GAPDH in the glycolysis pathway and was associated with relative mRNA-related angiogenesis and neurogenesis and increased expression. (A) Relative GAPDH mRNA expression of neural stem cells (NSCs) treated with metformin and iodoacetic acid (IA), as examined by qRT-PCR. (B) Relative VEGFR2 mRNA expression of NSCs treated with metformin and IA, as examined by qRT-PCR. (C) and (D), Relative Tuj1 (C) and GFAP (D) mRNA expression of NSCs treated with metformin and IA, as examined by qRT-PCR. Ctrl: Control; Met: Metformin. The overall significance between two groups was determined by Student’s t-test, among three group was determined by one-way ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001, ns, not significant.