Research Paper Volume 12, Issue 18 pp 18384—18395

Figure 3. p38γ shRNA provokes apoptosis in human OS cells. Human OS cells, including OS1/OS2/OS3 primary cells and the established U2OS cells, with scramble control shRNA (“shC”) or the applied p38γ shRNA (p38γ-shRNA-s1/s2), were cultured for applied time periods, the relative caspase-3 activity was tested (A, E), with expression of apoptosis-associated proteins examined by Western blotting (B); Cell apoptosis was tested by Annexin V FACS (C) and nuclear TUNEL staining (D, F) assays, and results were quantified. Expression of listed proteins was quantified and normalized to the loading control (B). Data presented as mean ± standard deviation (SD, n=5). *** p< 0.001 vs. “shC” cells. Experiments in this figure were repeated five times. Bar=100 μm (D).