Research Paper Volume 13, Issue 6 pp 9119—9134

Silencing of circular RNA_0000326 inhibits cervical cancer cell proliferation, migration and invasion by boosting microRNA-338-3p-dependent down-regulation of CDK4

miR-338-3p binds to circ

Figure 4. miR-338-3p binds to circ_0000326. (A) The potential binding site between circ_0000326 and miR-338-3p predicted by database. (B) The binding relationship between circ_0000326 and miR-338-3p detected by dual-luciferase reporter gene assay. * p < 0.05 vs. NC. (C) RTq-PCR analysis of miR-338-3p following RNA pull-down assays with circ-0000326 probes in SiHa and Hela cells. * p < 0.05. (D) Circ_0000326 co-precipitation with Ago2 using RIP, * p < 0.05 vs. IgG. (E) RTq-PCR analysis of miR-338-3p expression after sh-circ-0000326, * p < 0.05 vs. NC. Data were expressed as mean ± standard deviation and analyzed by unpaired t test.