Figure 5. Circ_0000326 promotes CDK4 expression by functioning as a sponge of miR-338-3p. (A) CDK4 expression in cervical cancer tissues predicted by TCGA database. (B) Representative images of immunohistochemistry (× 200) and positive-CDK4 ratio. (C) RT-qPCR analysis of CDK4 expression in 60 paired cervical cancer tissues and adjacent normal tissues. (D) The binding relationship between CDK4 and miR-338-3p detected using dual-luciferase reporter assay. (E, F) CDK4 mRNA expression in SiHa and Hela cells after alteration of miR-338-3p and silencing of circ_0000326 measured by RT-qPCR. (G–I) CDK4 protein expression in SiHa and Hela cells after treatment of circ_0000326 detected by Western blot analysis. Data were expressed as mean ± standard deviation. The data between two groups were analyzed by unpaired t-test with independent sample while the data among multiple groups was analyzed by ANOVA followed by Tukey’ s post hoc test.