Research Paper Volume 13, Issue 6 pp 9119—9134

Silencing of circular RNA_0000326 inhibits cervical cancer cell proliferation, migration and invasion by boosting microRNA-338-3p-dependent down-regulation of CDK4

Circ

Figure 6. Circ_0000326 promotes cervical cancer cell proliferation by targeting miR-338-3p. (A) Representative images of clone formation assay upon treatment with NC, miR-338-3p-mimic, miR-338-3p-inhibitor or sh-circ_0000326 + miR-338-3p-inhibitor. (B) Cervical cancer cell proliferation measured by EdU (× 200). (C) Cervical cancer cell proliferation rates measured by EdU. (D) OD value of cervical cancer cells determined by CCK-8. (E) The protein expression of cell cycle-related gene using Western blot analysis. * p < 0.05. vs. SiHa and Hela cells treated with NC. Data were expressed as mean ± standard deviation. The data between two groups were analyzed by unpaired t-test with independent sample while the data among multiple groups was analyzed by ANOVA. Data at different time points were analyzed by Two-Way ANOVA.