Research Paper Volume 12, Issue 17 pp 17343—17352

High hsa_circ_0020123 expression indicates poor progression to non-small cell lung cancer by regulating the miR-495/HOXC9 axis


Figure 2. Downregulation of hsa_circ_0020123 suppresses tumor formation in nude mouse xenografts. (A) qRT-PCR shows expression of hsa_circ_0020123 in adenovirus-transfected (sh-hsa_circ_0020123) and circRNA control-transfected (sh-NC) A549 and H1299 cell lines. (B) Representative images of nude mouse xenografts of A549 cells (n = 6). (C) Tumor volumes in mice were measured every 5 days. Data are means ± SD. ***P < 0.001 vs. controls. (D) Tumor weight was measured 30 days after grafting. Data are the means ± SD. ***P < 0.001 vs. controls. (E) Immunohistochemical staining of Ki67 in tumor tissues from both sh-NC and sh-circRNA groups.(F) qRT-PCR assay of miR-495 expression. Data are means ± SD. ***P < 0.001 vs. controls. (G, H) Western blot assay of HOXC9 protein expression in tumor tissues. Data are means ± SD. ***P < 0.001 vs. controls.