Research Paper Volume 12, Issue 17 pp 17353—17366

Taurine suppresses ROS-dependent autophagy via activating Akt/mTOR signaling pathway in calcium oxalate crystals-induced renal tubular epithelial cell injury

Figure 1. Tau ameliorated CaOx crystals-induced HK-2 cell injury. (A) Effect of Tau on the viability of HK-2 cells. (B) Effect of Tau on the viability of cells exposed to CaOx crystals. (C) The concentration of secreted LDH in cell supernatants. (D) The apoptosis of HK-2 cells was assessed using TUNEL assay; scale bar: 50 μm. (E) ELISA detection of IL-1β expression in culture supernatants. (F) Observation of the crystal adhesion on HK-2 cells under a light microscope. Red arrows indicate intracellular CaOx crystals; scale bar: 20 μm. Data are presented as the mean ± SD (n=3). *P < 0.05 versus the control group, #P < 0.05 versus the CaOx group.