Research Paper Volume 12, Issue 17 pp 17367—17379

G-protein coupled estrogen receptor activation protects the viability of hyperoxia-treated primary murine retinal microglia by reducing ER stress

Figure 1. Basic characterization of primary murine retinal microglia. (A) The representative images (100×, Bar=10μm) show in vitro cultured primary retinal microglia cells on days 5 (D5), 10 (D10) and 14 (D14). (B) Representative confocal fluorescence microscopic images (400×; Bar=25μm) show Alexa Fluor 488-tagged anti-CD11c antibody (green) staining of primary murine retinal microglia. The negative control cells are treated with PBS instead of the primary anti-CD11c antibody. (C) Representative confocal fluorescence microscopic images (400×; Bar=25μm) show Alexa Fluor 488-tagged anti-GPER antibody (green) staining of primary murine retinal microglia. The negative control cells are treated with PBS instead of the primary anti-GPER antibody.