Research Paper Volume 12, Issue 17 pp 17367—17379

G-protein coupled estrogen receptor activation protects the viability of hyperoxia-treated primary murine retinal microglia by reducing ER stress

Figure 5. GPER activation reduces induction of ER-stress related proteins in hyperoxia-treated primary retinal microglia. (A) Representative western blot images and (B) Histogram plots show the levels of IRE1α, PERK and ATF6 proteins in the (a) control (C), (b) hyperoxia (H), (c) hyperoxia+DMSO (DMSO), (d) hyperoxia+G-1 (G-1), and (e) hyperoxia+G-1+G-15 (G-1+G-15) groups of primary retinal microglia at 12h, 24h, 48h and 72h. β-actin was used as internal control. P<0.05 denotes statistical significance. The experiments were repeated thrice and means±S.D are shown.