Figure 10. Invitro breast cancer cell line experiments validate the predictive value of the eight-gene signature for immune checkpoints. (A) Raw data (Ct value) from Rt-PCR analysis of the eight genes in four breast cancer cell lines and one normal breast cell line. (B) Risk scores of the four breast cell lines calculated based on Rt-PCR results. Western blot results for (C) PD1, (D) OX40, (E) B7H3, (F) LAG-3, and (G) PDL1 expression in the four breast cancer cell lines.