Research Paper Volume 12, Issue 17 pp 17568—17581

HSPB8 overexpression prevents disruption of blood-brain barrier after intracerebral hemorrhage in rats through Akt/GSK3β/β-catenin signaling pathway

Figure 2. Lentivirus i.c.v administration mediated HSPB8 overexpression in brain tissues. (A) Representative fluorescent images labeled with anti-green fluorescent protein (GFP). a1, b1, and c1 were images at lower magnification in the hippocampus, striatum and cortex; a2, b2, and c2 were at higher magnifications. (B) RT-PCR result analysis (n=5, mean±SEM). (C) Western blotting and quantification of HSPB8 protein (n=5, mean±SEM). (D) Representative photomicrographs of immunostaining for HSPB8 (red) and DAPI (blue). Scale bars = 50 μm. **P<0.01 versus pLV-GFP. n=number of animals per group.