Figure 5. HSPB8 overexpression reduced neutrophil infiltration and proinflammatory cytokine production at 24h following ICH. (A) Representative pictures of infiltrated neutrophils (MPO + cells) in peri-hematoma area of ipsilateral cortex 24h after perfusion following ICH. Nuclei were stained with DAPI. Scale bar = 50μm (n=5, mean ± SEM). (B, C). IL-1β and TNF-α secretions in perihematomal area were analyzed by ELISA (n=5, mean ± SEM). (D, E). mRNA of IL-1β and TNF-α in perihematomal area were analyzed by RT-PCR (n=5, mean ± SEM). **P <0.01 versus indicated group. n=number of animals per group.