Figure 7. HSPB8 activated Akt/GSK3β pathway and Akt inhibition abolished the restorative effect of HSPB8 on TJPs. (A) Western blot analysis was performed to measure the protein levels of HSPB8, p-Akt, Akt, p-GSK3β, GSK3β, occludin and claudin-5. (B–F) Quantitative analysis of western blot analyses (n=6, mean± SEM). *P <0.05 versus indicated group; **P <0.01 versus indicated group. n=number of animals per group.