Research Paper Volume 12, Issue 17 pp 17601—17624

Figure 7. Ultrastructure of newly generated podocytes from PEC origin (tdTomato⁺EGFP⁺) in the glomerular tuft of aged mice. (A) Representative confocal image of expansion microscopy of a glomerulus in an aged mouse with a newly generated podocyte from PEC origin (marked with dashed box). Superscripts show separate channels: tdTomato⁺ (red) (A¹) and EGFP⁺ (A²). (B) Higher magnification of image shown in A of newly generated podocyte, with accompanied single color images of tdTomato (B¹) and EGFP (B²). (C) The newly generated podocyte has classic podocyte architecture: a cell body (labeled with red circle), primary process (red triangle), several secondary processes (red rhombuses), branching to minor and tertiary foot processes (C¹–C³ labeled with dashed boxes). (D–F) Higher magnification of images shown in dashed boxes C¹–C³ with different z-stack focal planes show various tertiary foot processes (labeled with red arrows). Scale bars represent 25μm or 5 μm (inset) and are in pre-expansion dimensions.