Figure 3. Lnc-EPIC1 siRNA provokes apoptosis in colon cancer cells. The primary human colon cancer cells, pri-Can-1/-2/-3, were transfected with scramble control siRNA (“scr”, 500 nM) or the applied Lnc-EPIC1 siRNA (si-Lnc-EPIC1-s1/si-Lnc-EPIC1-s2, 500 nM, for 48h), cells were further cultured for additional 48h, expression of listed proteins in total cell lysates was tested by Western blotting assays (A), the relative caspase-3 activity was tested (B, F), with mitochondrial depolarization examined by JC-1 staining (C); Cell apoptosis was examined and quantified by nuclear TUNEL staining (D, G) and Annexin V FACS (E). Data presented as mean ± standard deviation (SD). * p< 0.05 vs. “scr” cells. Experiments in this figure were repeated five times. Bar=100 μm (C, D).