Research Paper Volume 13, Issue 8 pp 12207—12223

Anti-oncogenic effects of SOX2 silencing on hepatocellular carcinoma achieved by upregulating miR-222-5p-dependent CYLD via the long noncoding RNA CCAT1


Figure 2. Silencing of SOX2 and CCAT1 decreases EGFR expression to suppress HepG2 cell proliferation, migration, and invasion. HepG2 cells were treated with sh-NC + oe-NC, sh-SOX2 + oe-NC, sh-CCAT1 + oe-NC, sh-SOX2 + oe-EGFR or sh-CCAT1 + oe-EGFR. (A) mRNA expression of EGFR in HepG2 cells detected by RT-qPCR normalized to β-actin. (B) Cell viability determined by MTT assay. (C, D) cell migration determined by Transwell assay (200 ×). (E, F) Cell invasion determined by Transwell assay (200 ×). * p < 0.05 vs. HepG2 treated with sh-NC + oe-NC. # p < 0.05 vs. HepG2 treated with sh-SOX2 + oe-NC. & p < 0.05 vs. HepG2 treated with sh-SOX2 + oe-NC. Data were expressed as mean ± standard deviation. Data from multiple groups were compared by one-way ANOVA and Tukey’s post hoc test. Data were compared between groups at different time points by repeated measures ANOVA and Bonferroni-corrected post hoc testing.