Research Paper Volume 13, Issue 8 pp 12207—12223

Anti-oncogenic effects of SOX2 silencing on hepatocellular carcinoma achieved by upregulating miR-222-5p-dependent CYLD via the long noncoding RNA CCAT1


Figure 6. SOX2 downregulation declines cell proliferation, migration, and invasion via miR-222-5p/CYLD in HepG2 cells. HepG2 cells were treated with sh-NC + mimic-NC, sh-SOX2 + mimic-NC, or sh-SOX2 + miR-222-5p mimic. (A) CYLD mRNA expression in HepG2 cells detected by RT-qPCR normalized to β-actin. (B). CYLD protein expression in HepG2 cells determined by western blot analysis normalized to GAPDH. (C) Cell viability determined by MTT assay. (D, E) Cell migration determined by Transwell assay (200 ×). (F, G) Cell invasion determined by Transwell assay (200 ×). * p < 0.05 vs. HepG2 treated with sh-NC + mimic-NC. # p < 0.05 vs. HepG2 treated with sh-SOX2 + mimic-NC. Data are expressed as mean ± standard deviation. Data from multiple groups were compared by one-way ANOVA and Tukey’s post hoc test. Data were compared between groups at different time points by repeated measures ANOVA and Bonferroni post hoc test.