Activation of TLR3 signaling by Au-antimiR-155 NCs. (A) Western blotting analysis of TLR3, NF-αB (p65), and both full-length and cleaved caspase-8 expression in HepG2 cells treated with antimiR-155, gold precursor plus antimiR-155, or the respective controls (n=3). (B) Densitometric quantification of data shown in (A). (C) qRT-RCR analysis of TLR3, p65, and caspase-8 expression in HepG2 cells (n=3). (D) Quantification of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) secretion by ELISA. HepG2 cells were co-incubated with gold precursor and antimiR-155 for 24 h. **P< 0.01; *P < 0.05; ns, no significant difference.