Research Paper Volume 12, Issue 18 pp 18501—18521

D-galactose induces senescence of glioblastoma cells through YAP-CDK6 pathway

Figure 6. D-gal induced senescence of GBM cells by inactivating YAP-CDK6 pathway in a Hippo pathway dependent manner. (A) Western blot detected the protein expression of p-YAP, YAP, p-MST1, MST1, p-LATS1, LATS1, CDK6, p27kip1, and Lamin B1 in control and senescent C6 cells (treated with 222 mM D-gal for 8 d). (BI) Quantification of protein levels of p-YAP/YAP, YAP, p-MST1/MST1, MST1, p-LATS1/LATS1, LATS1, CDK6 and p27kip1 (n=3) level as shown in (A). Data shown are mean ± s.e.m. *P < 0.05, **P < 0.01.