Research Paper Volume 12, Issue 17 pp 17634—17646

DUSP4 directly deubiquitinates and stabilizes Smad4 protein, promoting proliferation and metastasis of colorectal cancer cells

Figure 2. DUSP4 promoted metastasis and proliferation of colorectal cancer cells in vitro. (A) Western blot analysis of DUSP4 expression in FHC, LOVO, SW480, SW620, HCT116, and DLD1. (B) qRT-PCR analysis of DUSP4 expression in FHC, LOVO, SW480, SW620, HCT116, and DLD1. (C) Knockdown treatment of three designed siRNAs in SW480 cells. (D) DUSP4 protein expression of DUSP4 knockdown-treated SW480 cells and DUSP4 overexpression-treated HCT116 cells. (E) Cell proliferation analysis of DUSP4 knockdown-treated SW480 cells. (F) Cell proliferation analysis of DUSP4 overexpression-treated HCT116 cells. (G) Colony formation analysis of DUSP4 knockdown-treated SW480 cells and DUSP4 overexpression-treated HCT116 cells. (H) Western blot analysis of cell proliferation-related biomarkers expression in DUSP4 knockdown-treated SW480 cells and DUSP4 overexpression-treated HCT116 cells. **P<0.01, ***P<0.001.