Research Paper Volume 12, Issue 17 pp 17634—17646

DUSP4 directly deubiquitinates and stabilizes Smad4 protein, promoting proliferation and metastasis of colorectal cancer cells

Figure 3. Regulation of USP4 on colorectal cancer cell migration and invasion. (A) Cell scratch test of DUSP4 knockdown-treated SW480 cells. (B) Cell scratch test of DUSP4 overexpression-treated HCT116 cells. (C and D) Cell migration and invasion analysis of DUSP4 knockdown-treated SW480 cells and DUSP4 overexpression-treated HCT116 cells, respectively. (E) Western blot analysis of EMT-related biomarkers expression in DUSP4 knockdown-treated SW480 cells and DUSP4 overexpression-treated HCT116 cells. (F) qRT-PCR analysis of EMT-related biomarkers expression in DUSP4 knockdown-treated SW480 cells and DUSP4 overexpression-treated HCT116 cells. **P<0.01, ***P<0.001.