Research Paper Volume 12, Issue 17 pp 17634—17646

DUSP4 directly deubiquitinates and stabilizes Smad4 protein, promoting proliferation and metastasis of colorectal cancer cells

Figure 6. Rescue experiment. (A) CCK8 analysis of DUSP4 knockdown-treated SW480 cells and DUSP4 overexpression-treated HCT116 cells that had been treated with Smad 4 knockdown and overexpression treatments. (B) Western blot and qRT-PCR analysis of Cyclin D and PCNA expression in USP4 knockdown-treated SW480 cells and DUSP4 overexpression-treated HCT116 cells that had been treated with Smad 4 knockdown and overexpression treatments. (C and D) Migration and invasion analysis of DUSP4 knockdown-treated SW480 cells that had been treated with Smad4 overexpression treatment. (E and F) Migration and invasion analysis of DUSP4 overexpression-treated HCT116 cells that had been treated with Smad 4 knockdown treatment. (G and H) qRT-PCR analysis of EMT related biomarkers in DUSP4 knockdown-treated SW480 cells and DUSP4 overexpression-treated HCT116 cells that had been treated with Smad 4 knockdown and overexpression treatments. **P<0.01, ***P<0.001.