Research Paper Volume 12, Issue 18 pp 18522—18544

GPA peptide inhibits NLRP3 inflammasome activation to ameliorate colitis through AMPK pathway

Figure 2. GPA relieved DSS-induced colitis in a NLRP3-dependent manner. Wild-type (WT) and NLRP3-/- mice were treated with 3% DSS in their drinking water for 7 days to induce acute colitis. GPA (100mg/kg) was administered for 14 days before and during DSS treatment via oral gavage once per day. Mice were sacrifced at day 14 (n=10 mice/group). Percentage survival of mice after DSS treatment (A). Weight change of mice during the experiment (B). The lengths of colons from each group of mice were measured (C). H&E stains of serial sections of colons, histopathological scores of each group were determined (D). Myeloperoxidase (MPO) activity in the colonic tissues was detected (E). Data are presented as mean ± SD, n=10 /group. *p < 0.05, **p < 0.01 and ***p < 0.001.