Figure 3. GPA inhibited NLRP3 inflammasome assembly and GSDMD cleavage. THP-1 cells were primed with LPS for 4h, followed by GPA treatment 6 h before stimulation with ATP for 30 min. Immunoblot analyzed of ASC oligomerization in lysates of THP-1 cells (A). IP and immunoblot analyzed of the interaction of endogenous NLRP3 and ASC in THP-1 cells (B). Immunoblot analyzed of NLRP3 by SDD-AGE assay in THP-1 cells (C). Immunoblot analyzed of cleavage of GSDMD in THP-1 cells (D). IP and immunoblot analyzed of the interaction of endogenous NLRP3 and GSDMD in THP-1 cells (E). Three independent experiments.