Research Paper Volume 13, Issue 11 pp 15638—15658

Transfer of exosomal microRNA-203-3p from dendritic cells to bone marrow-derived macrophages reduces development of atherosclerosis by downregulating Ctss in mice

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Figure 4.

Overexpression of miR-203-3p in DCexs impairs AS-related phenotypes in BMDMs. BMDMs were treated with miR-203-3p mimic with NC mimic as the control, or treated with DCex-miR-203-3p inhibitor with DCex-NC inhibitor as control. (A and D) The proportion of foam cells in BMDMs by oil red O staining (200 ×). (B and E) Serum TC, FC and CE levels determined by ELISA. (C and F) miR-203-3p expression measured by RT-qPCR; * p < 0.05, ** p < 0.01, *** p < 0.001 vs. the BMDMs treated with NC mimic or DCexs treated with NC inhibitor. Statistical data were measurement data, and described as mean ± standard deviation. The paired t test was used for comparisons between two groups. The experiment was repeated 3 times independently.