Figure 2. SNHG22 influenced the proliferation and migration of OS cells. (A, B) The qRT-PCR measured the transfection efficiency of SNHG22 in U2OS and HOS cells. n=5; ***P<0.001 by t test. (C, D) Overexpression of SNHG22 reduced the cell viability of U2OS and HOS cells while SNHG22 knockdown showed the reverse effect. Cell viability and proliferation were assessed by CCK-8 assays and western blots. n=5; *P<0.05, **P<0.01. (E, F) Colony formation of HOS and U2OS cell lines was synergistically suppressed by overexpression of SNHG22 and was obviously enhanced by SNHG22 silencing. The number of clones is shown in the violin plot. n=5; **P<0.01, ***P<0.001 by t-test. (G, H) Cell migration rates of U2OS and HOS cells were measured by wound healing assays; scale bar: 100 μm. n=5; *P<0.05, **P<0.01, ***P<0.001 by t-test.