Research Paper Volume 12, Issue 18 pp 18571—18587

Characterization of LncRNA SNHG22 as a protector of NKIRAS2 through miR-4492 binding in osteosarcoma

Figure 4. SNHG22 regulated endothelial cell function. (A) Representative images of HUVEC wound healing assays in the presence of U2OS supernatant. Scale bar: 100 μm. (B) Relative cell migration rates of HUVECs were measured. n=5; *P<0.05 by t-test. (C) Cell viability of HUVECs was assessed by CCK-8 assay. n=5; *P<0.05, **P<0.01. (D) Representative images of invading HUVECs. U2OS supernatant was included in all cultures. Scale bar: 200 μm. (E) The number of invading HUVECs were counted and are shown in the violin plots. n=5; **P<0.01 by t-test. (F) The level of VEGF in U2OS supernatant. Results are shown in the violin plots. n=5; ***P<0.001 by t-test. (G) Representative images of HUVEC wound healing assays in the presence of HOS supernatant. Scale bar: 100 μm. (H) Relative cell migration rates of HUVECs were measured. n=5; *P<0.05, **P<0.01 by t-test. (I) Cell viability of HUVECs was assessed by CCK-8 assays. n=5; *P<0.05, **P<0.01. (J) Representative images of invading HUVECs. HOS supernatant was included in all cultures. Scale bar: 200 μm. (K) The number of invading HUVECs were counted and are shown in the violin plots. n=5; **P<0.01, ***P<0.001 by the t-test. (L) The level of VEGF in HOS supernatant. Results are shown in the violin plots. n=5; ***P<0.001 by the t-test.