Research Paper Volume 12, Issue 18 pp 18571—18587

Characterization of LncRNA SNHG22 as a protector of NKIRAS2 through miR-4492 binding in osteosarcoma

Figure 6. SNHG22 was associated with miR-4492 in OS cells. (A) Constructs containing SNHG22 transcripts were designed and inserted into MS2bs elements. MS2-RIP was performed, and miR-4492 qPCR was carried out to verify that miR-4492 endogenously associated with SNHG22. n=5; *P<0.05 by t-test. (B) Plasmids containing mutant or putative SNHG22 3′-UTR-luciferase reporters were transfected into U2OS and HOS cells. (C) Luciferase activity in U2OS and HOS cells was detected and is shown as means±SD. n=5; *P<0.05, **P<0.01 by the t-test. (D) After incubating with biotin-labeled miR-4492, the expression level of SNHG22 was detected by qPCR. n=5; ***P<0.001 by the t-test. (E) Cell viability of U2OS and HOS cells was assessed by CCK-8 assays. n=5; *P<0.05, **P<0.01. (F) Representative images of invading cells in mimic NC, mimic miR-4492, inhibitor NC, inhibitor miR-4492, inhibitor NC +PLKO.1-VECTOR, inhibitor NC +PLKO.1-SNHG22, inhibitor miR-4492+PLKO.1-VECTOR and inhibitor miR-4492+PLKO.1-SNHG22 groups. Scale bars: 200 μm. (G) The number of invading cells is shown in the violin plot. n=5; **P<0.01 and ***P<0.001 by the t-test.