Research Paper Volume 12, Issue 18 pp 18588—18602

Lipopolysaccharide upregulates miR-132/212 in Hirschsprung-associated enterocolitis, facilitating pyroptosis by activating NLRP3 inflammasome via targeting Sirtuin 1 (SIRT1)

Figure 2. SIRT1 dysregulation by LPS as a result of miR-132 and miR-212 overexpression. (A) SIRT1 expression was tested to be down-regulated in dilated segments of 32 po-HAEC compared with 32 No-HAEC patients by qRT-PCR. As revealed by western blot analysis, SIRT1 protein level was declined in 4 pairs of po-HAEC tissues compared to No-HAEC samples. (B) In comparison with control mice group, SIRT1 mRNA level was decreased in LPS-stimulated mice intestinal tissues. The protein expression of SIRT1 was notably decreased in animal models treated with LPS. (C) SIRT1 was inversely correlated with miR-132 and miR-212 levels in the same paired dilated tissues (P=0.0454, P=0.0474, Pearson). (D) Both mRNA and protein levels of SIRT1 were down-regulated in HT29 cell line after treatment with LPS for 24h. Mean ± SD. n = 3, *P < 0.05.