Research Paper Volume 13, Issue 8 pp 12239—12257

Extracellular-vesicle containing miRNA-503-5p released by macrophages contributes to atherosclerosis

Expression patterns of miR-503-5p, smad7, smurf1, and smurf2 in plasma-EVs of patients with atherosclerosis. (A, B) Expression of miR-503-5p (normalized to U6), TGF-β1, smad7, smurf1, and smurf2 (all normalized to GAPDH) in plasma-EVs of healthy individuals (n = 30) and patients with atherosclerosis (n = 53) determined by RT-qPCR. (C) Pearson analyses of miR-503-5p and TGF-β1, smad7, smurf1, or smurf2. Values obtained from three independent experiments in triplicate were analyzed by one-way ANOVA followed by Tukey's post hoc test among three or more groups. *p

Figure 1. Expression patterns of miR-503-5p, smad7, smurf1, and smurf2 in plasma-EVs of patients with atherosclerosis. (A, B) Expression of miR-503-5p (normalized to U6), TGF-β1, smad7, smurf1, and smurf2 (all normalized to GAPDH) in plasma-EVs of healthy individuals (n = 30) and patients with atherosclerosis (n = 53) determined by RT-qPCR. (C) Pearson analyses of miR-503-5p and TGF-β1, smad7, smurf1, or smurf2. Values obtained from three independent experiments in triplicate were analyzed by one-way ANOVA followed by Tukey's post hoc test among three or more groups. *p < 0.05 compared with healthy individuals.