Research Paper Volume 12, Issue 18 pp 18635—18648

Visfatin increases ICAM-1 expression and monocyte adhesion in human osteoarthritis synovial fibroblasts by reducing miR-320a expression

Figure 7. Visfatin suppresses miR-320a synthesis via the AMPK and p38 pathways. (A) Schematic 3′-UTR representation of human ICAM-1 containing the miR-320a binding site. (B) OASFs were transfected with the indicated luciferase plasmid with or without miR-320a mimic, then stimulated with visfatin. Relative luciferase activity was examined. (C) OASFs were pretreated with Ara A, compound C and SB203580 for 30 min, then incubated with visfatin for 24 h. The expression of miR-320a was examined by qPCR. * p<0.05 compared with the control group; #p<0.05 compared with the visfatin-treated group.