Research Paper Volume 12, Issue 16 pp 16621—16646

Prolonged treatment with Y-27632 promotes the senescence of primary human dermal fibroblasts by increasing the expression of IGFBP-5 and transforming them into a CAF-like phenotype

Figure 5. Identification of IGFBP-5 as a potential factor in the decreased growth of HDFs treated with Y-27632. (A, B) HDFs were cultured in growth medium in the presence of 10 μM Y-27632 or PBS (as a control) for 12 and 48 h, then were collected for the isolation of total RNAs for RNA-seq. KEGG pathway enrichment analysis of DEGs, and the top 20 pathways are shown for each time point (left panel for 12 h, right panel for 48 h) in (A). The size of each dot represents the degree of enrichment and the color indicates the degree of statistical significance. A Biological Process map of GO enrichment analysis is shown in (B). Genes related with ‘negative regulation growth’ from Gene Ontology (GO) enrichment analysis are listed in the table expanded on the right.