Figure 3. Knockdown of TXNDC9 promoted autophagy in U87 cells. (A) The level of GFP-LC3 and localization in U87 and U251 cells after transfecting with si-TXNDC9/si-NC. Representative immunofluorescence images were shown. (B) The protein level of Beclin-1 and LC3-I/II was detected in U87 and U251 cells, Gapdh was indicated as a loading control. n= 6, *P<0.05.