Figure 5. TXNDC9 regulated glioma program via controlling p53. (A) The colony formation assay. (B, C) Wound healing assay and transwell were performed for detecting the effect of p53 on migration and invasion. (D) The apoptosis rate of U87 and U251 cells was measured after si-TXNDC9/si-NC transfection and PFTα treatment. The histogram at the right is a statistical graph. n=6, *P<0.05. (E) The protein level of p53, Cleaved-caspase3, Cleaved-caspase8, and Cleaved-caspase9 were detected by western blot, Gapdh was indicated as a loading control. n= 6, *P<0.05.