Figure 6. miR-133b confers protection against neuronal cell apoptosis induced by hypoxia through inhibition of JAK1. (A) miR-133b expression profile and JAK1 mRNA level determined using RT-qPCR. (B) Protein levels of JAK1, p-AKT/AKT, p-GSK-3β/GSK-3β, and WNT-3 normalized to GAPDH determined using Western blot analysis. (C) Apoptosis of neuronal cells assessed by TUNEL staining (× 200). (D) Cell viability detected by CCK-8 assay. * p < 0.05 vs. the mimic NC + pcDNA-NC group (neuronal cells treated with mimic NC + pcDNA-NC); # p < 0.05 vs. the miR-133b mimic + pcDNA-NC group (neuronal cells treated with miR-133b mimic + pcDNA-NC). Data obtained from three independent cell experiments were expressed as mean ± standard deviation and data among multiple groups were analyzed by one-way ANOVA followed by the Tukey's post hoc test.