Priority Research Paper Volume 12, Issue 18 pp 17786—17799

Rapamycin maintains NAD+/NADH redox homeostasis in muscle cells

Figure 6. Optical redox imaging of old mouse muscles. (A, B) Representative white light and redox images of one layer of a quadriceps specimen from control (A) and treated (B) groups. The in-plane spatial resolution of the redox images is 100 μm. The concentrations of Fp and NADH are nominal concentrations in reference to the embedded flavin adenine dinucleotide (FAD) and NADH standards, respectively. The color bars for Fp and NADH images are nominal concentrations in reference to the embedded standards, respectively, and that for the redox ratio image ranges from 0-0.4. (C) Scattered plots of redox indices (Fp, NADH, and the redox ratio) for all specimens comparing control and treated groups. Each dot is a mean value for a specific redox index of a tissue layer and the tissue layers from the same tissue specimen are encoded with the same color (bars: median with 95% CI).